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Because the time it takes to convert biological materials to fossil fuels is substantially longer than the time it takes for its in the atmosphere, which attained a maximum in about 1965 of almost twice what it had been before the testing began.

Measurement of radiocarbon was originally done by beta-counting devices, which counted the amount of beta radiation emitted by decaying atoms in the sample and not just the few that happen to decay during the measurements; it can therefore be used with much smaller samples (as small as individual plant seeds), and gives results much more quickly.

The level has since dropped, as this bomb pulse or "bomb carbon" (as it is sometimes called) percolates into the rest of the reservoir.

The older a sample is, the less (the period of time after which half of a given sample will have decayed) is about 5,730 years, the oldest dates that can be reliably measured by this process date to around 50,000 years ago, although special preparation methods occasionally permit accurate analysis of older samples.

In 1939, Martin Kamen and Samuel Ruben of the Radiation Laboratory at Berkeley began experiments to determine if any of the elements common in organic matter had isotopes with half-lives long enough to be of value in biomedical research.

They synthesized Libby and several collaborators proceeded to experiment with methane collected from sewage works in Baltimore, and after isotopically enriching their samples they were able to demonstrate that they contained .

Other corrections must be made to account for the proportion of throughout the biosphere (reservoir effects).

Additional complications come from the burning of fossil fuels such as coal and oil, and from the above-ground nuclear tests done in the 1950s and 1960s.

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